Team:Chiba/Notebook/Calendar/3 September 2009

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Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 2)

  • main culture

10:30 We took 30 mL of the liquid medium that we had made yesterday to conical flask and added prior culture solution 300 μL, then started shake culture at 37 degrees Celsius.

  • Making glycerol stock

We kept mixture of 300 μL of 50% glycerol and 300 μL of solution which is cultured yesterday in deep freezer.


  • Making Transfer Curves

16:00

We put the solution that has completed main culture in 48 deep wells.

17:15

We shook 48 deep wells at 30 degrees Celsius.

While shaking, we measured OD and GFP fluorescence intensity of sample.


OD

0 μM AHL 0.597 0.590 0.589 0.043(control)
100 μM AHL 0.592 0.595 0.591


GFP Fluorescence Intensity

0 μM AHL 2.218 2.043 2.099 1.748(control)
100 μM AHL 2.139 2.114 2.121


18:30

We put the sample on plate and measured OD and GFP fluorescence intensity.

OD

0.624 0.635 0.613 0.040
0.631 0.617 0.601


GFP Fluorescence Intensity

2.119 2.035 2.038 1.718
2.044 2.183 2.105


19:24

In a similar way.

OD

0.741 0.724 0.717 0.040
0.718 0.698 0.678


GFP Fluorescence Intensity

2.071 2.050 2.102 1.666
2.449 2.263 2.344


20:23

In a similar way.

OD

0.774 0.742 0.742 0.040
0.735 0.723 0.760


GFP Fluorescence Intensity

2.158 2.227 2.364 1.646
2.815 2.767 2.756


21:18

In a similar way.

OD

0.861 0.813 0.825 0.039
0.755 0.818 0.849


GFP Fluorescence Intensity

2.062 2.775 2.673 1.814
3.615 3.510 3.453


22:56

We stopped this experiment.


Handwritten Lab Notebook

Chi 20090903 1.JPG

Chiba Labwork 03Sep09.jpg Chiba Labwork 03Sep09-2.jpg