Team:Chiba/Notebook/protocol
From 2009.igem.org
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!align="center"|[[Team:Chiba/Contact|Contact]] | !align="center"|[[Team:Chiba/Contact|Contact]] | ||
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+ | == Protocols == | ||
+ | *Transformation (Using Zymo comp.) | ||
+ | *Electro poration | ||
+ | *DNA Purification | ||
+ | :*Sigma prep | ||
+ | :*Zymo DNA Cleam&Concentrator Kit | ||
+ | *Agarose gel electrophoresis | ||
+ | *Digestion | ||
+ | *PCR | ||
+ | *Gel extract | ||
+ | *Dephosphorylation of DNA | ||
+ | *Phosphorylation of DNA | ||
+ | *Ligation | ||
+ | *Time Delay Test | ||
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+ | ===DNA Purification=== | ||
+ | ====Sigma prep==== | ||
+ | ====Zymo DNA Cleam&Concentrator Kit==== | ||
+ | |||
+ | ===Agarose gel electrophoresis=== | ||
+ | ===Digestion=== | ||
+ | ===PCR=== | ||
+ | ===Gel extract=== | ||
+ | ===Dephosphorylation of DNA=== | ||
+ | ===Phosphorylation of DNA=== | ||
+ | ===Ligation=== | ||
+ | ===Time Delay Test=== | ||
Revision as of 07:37, 28 August 2009
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Protocols
TransformationDay 1 morning
Day 1 night
Day 2
DNA PurificationSigma prepZymo DNA Cleam&Concentrator KitAgarose gel electrophoresisDigestionPCRGel extractDephosphorylation of DNAPhosphorylation of DNALigationTime Delay Test |