Team:Chiba/Notebook/protocol
From 2009.igem.org
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===Gel extract=== | ===Gel extract=== | ||
- | READ:[[Team:Chiba/protocol#Agarose_gel_electrophoresis]] | + | READ:[[Team:Chiba/Notebook/protocol#Agarose_gel_electrophoresis|Agarose_gel_electrophoresis]] |
#Run the Digested DNA solution | #Run the Digested DNA solution | ||
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#Purify the DNA with Zymo DNA Cleam&Concentrator Kit | #Purify the DNA with Zymo DNA Cleam&Concentrator Kit | ||
- | READ:[[Team:Chiba/protocol#Zymo_DNA_Cleam&Concentrator_Kit]] | + | READ:[[Team:Chiba/Notebook/protocol#Zymo_DNA_Cleam&Concentrator_Kit|Zymo_DNA_Cleam&Concentrator_Kit]] |
- | + | ||
===Dephosphorylation of DNA=== | ===Dephosphorylation of DNA=== |
Revision as of 07:48, 28 August 2009
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Protocols
TransformationDay 1 morning
Day 1 night
Day 2
DNA PurificationSigma prepZymo DNA Cleam&Concentrator Kit
Agarose gel electrophoresis
DigestionPCR
Start: 94 °C for 5 min. (melt) cycle: melt: 1 min. anneal : 30 sec. cycle end: extension:72 °C for 3.5 min. 25 cycles 72 °C for 10 min store: keep at 6 °C forever
Gel extractREAD:Agarose_gel_electrophoresis
READ:Zymo_DNA_Cleam&Concentrator_Kit Dephosphorylation of DNAPhosphorylation of DNALigationTime Delay Test |