Team:Chiba/Notebook/protocol
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#[[Team:Chiba/Notebook/protocol#Phosphorylation_of_DNA|Phosphorylation of DNA]] | #[[Team:Chiba/Notebook/protocol#Phosphorylation_of_DNA|Phosphorylation of DNA]] | ||
#[[Team:Chiba/Notebook/protocol#Ligation|Ligation]] | #[[Team:Chiba/Notebook/protocol#Ligation|Ligation]] | ||
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== Protocols == | == Protocols == |
Revision as of 22:39, 21 October 2009
E.coli Time Manager -Since 2008-
Home | The Team | Parts | Reference | Notebook | Protocols | Links | Acknowledgements | Contact |
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Contents
ProtocolsTransformationDay 1 morning
Day 1 night
Day 2
DNA PurificationSigma prepZymo DNA Cleam&Concentrator Kit
Agarose gel electrophoresis
DigestionPCR
Start: 94 °C for 5 min. (melt) cycle: melt: 1 min. anneal : 30 sec. cycle end: extension:72 °C for 3.5 min. 25 cycles 72 °C for 10 min store: keep at 6 °C forever
Gel extract
Dephosphorylation of DNASAP: Alkaline Phosphatase (Shrimp)
Phosphorylation of DNALigationTime Delay Test
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