Team:Chiba/Parts

From 2009.igem.org

(Difference between revisions)
(Parts Status(Parts created by Chiba))
 
(8 intermediate revisions not shown)
Line 1: Line 1:
-
<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
+
<html><link rel="stylesheet" href="https://2009.igem.org/wiki/index.php?title=User:Yoshimi/chiba1.css&action=raw&ctype=text/css" type="text/css" /></html>
-
{| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Century Gothic', sans-serif;"
+
:::::<Font Size="7">'''''E''.coli Time Manager '''</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
 +
 
 +
 
 +
{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
 +
!align="center"|[[Team:Chiba|Home]]
 +
!align="center"|[[Team:Chiba/Team|The Team]]
 +
!align="center"|[[Team:Chiba/Parts|Parts]]
 +
!align="center"|[[Team:Chiba/Reference|Reference]]
 +
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 +
!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
 +
!align="center"|[[Team:Chiba/Links|Links]]
 +
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 +
!align="center"|[[Team:Chiba/Contact|Contact]]
 +
|}
 +
{| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Arial', sans-serif;"
|- style="background:#BC8F8FF; height:100px;" valign="top"
|- style="background:#BC8F8FF; height:100px;" valign="top"
|style="width:15%;"|
|style="width:15%;"|
-
<html><a href="https://2009.igem.org/Team:Chiba" target="_blank"><img src="
+
 
-
https://static.igem.org/mediawiki/2008/8/8c/Chiba-logo.gif" width="110" height="110" |right></a>
+
<table class="calendar" width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
-
</html>
+
-
<br>
+
-
<table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
+
<tr><td>
<tr><td>
'''[[Team:Chiba/Project|The Project]]'''
'''[[Team:Chiba/Project|The Project]]'''
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project#Introduction|Introduction]]
+
'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project#Project_Design|Project Design]]
+
'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project#Conclusions|Conclusions]]
+
'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
-
</td></tr></table>
+
-
 
+
-
 
+
-
<table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
+
-
<tr><td>
+
-
'''[[Team:Chiba/Project/Delay_Switch|Delay Switch]]'''
+
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project/Delay_Switch#Experiments_(2008)|Experiments 2008]]
+
3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project/Delay_Switch#Results_(2008)|Results 2008]]
+
3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project/Delay_Switch#Experiments_(2009)|Experiments 2009]]
+
3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
</td></tr><tr><td>
</td></tr><tr><td>
-
*[[Team:Chiba/Project/Delay_Switch#Results_(2009)|Results 2009]]
+
'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
-
</td></tr></table>
+
-
 
+
-
 
+
-
<table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
+
-
<tr><td>
+
-
'''[[Team:Chiba/Project/Parallel|Parallel Activation]]'''
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Modeling|Modeling]]
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Project/Parallel#Experiments|Experiments]]
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Project/Parallel#Results|Results]]
+
-
</td></tr></table>
+
-
 
+
-
 
+
-
<table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
+
-
<tr><td>
+
-
'''[[Team:Chiba/Project/Series|Series Activation]]'''
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Modeling|Modeling]]
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Project/Series#Experiments|Experiments]]
+
-
</td></tr><tr><td>
+
-
*[[Team:Chiba/Project/Series#Results|Results]]
+
</td></tr></table>
</td></tr></table>
|style="width:85%;" bgcolor="#FFFFFF"|
|style="width:85%;" bgcolor="#FFFFFF"|
-
<Font Size="6">''E''.coli Time Manager Since 2008</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
 
-
 
-
 
-
{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
 
-
!align="center"|[[Team:Chiba|Home]]
 
-
!align="center"|[[Team:Chiba/Team|The Team]]
 
-
!align="center"|[[Team:Chiba/Parts|Parts]]
 
-
!align="center"|[[Team:Chiba/Reference|Reference]]
 
-
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
-
!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
 
-
!align="center"|[[Team:Chiba/Links|Links]]
 
-
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
-
!align="center"|[[Team:Chiba/Contact|Contact]]
 
-
|}
 
__NOTOC__
__NOTOC__
==Parts Status(Parts created by Chiba)==
==Parts Status(Parts created by Chiba)==
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
<table width="100%" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
<tr>
<tr>
<td width="200">Part Name</td>
<td width="200">Part Name</td>
-
<td width="200">Sub Parts</td>
+
<td width="300">Sub Parts</td>
-
<td width="200">Part Length(bp)<td>
+
<td width="200">Plasmid Vector<td>
<td width="200">Plasmid Vector<td>
-
<td width="200">Origin<td>
 
<td width="300">Part Length(bp)<td>
<td width="300">Part Length(bp)<td>
<td width="200">Part and vector length(bp)<td>
<td width="200">Part and vector length(bp)<td>
<td width="200">Resistance<td>
<td width="200">Resistance<td>
-
<td width="200">Status<td>
+
<td width="50">Sequenced<td>
-
<td width="200">Sequenced<td>
+
<td width="50">Sent<td>
-
<td width="200">Sent<td>
+
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/Part:BBa_K247000 BBa_K247000]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_K247000 K247000]</td>
<td width="300">removed the enzyme site "Nco1"</td>
<td width="300">removed the enzyme site "Nco1"</td>
<td width="200">-<td>
<td width="200">-<td>
 +
<td width="300">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
-
<td width="200">-<td>
 
-
<td width="200">-<td>
 
-
<td width="200">-<td>
 
-
<td width="350">miniprepped<td>
 
<td width="50">no<td>
<td width="50">no<td>
<td width="50">no<td>
<td width="50">no<td>
Line 102: Line 63:
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/Part:BBa_K247001 BBa_K247001]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_K247001 K247001]</td>
<td width="300">mutant LuxR(Dull)</td>
<td width="300">mutant LuxR(Dull)</td>
<td width="200">-<td>
<td width="200">-<td>
 +
<td width="300">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
-
<td width="200">-<td>
 
-
<td width="200">-<td>
 
-
<td width="200">-<td>
 
-
<td width="350">-<td>
 
<td width="50">no<td>
<td width="50">no<td>
<td width="50">no<td>
<td width="50">no<td>
Line 118: Line 76:
<td width="300">plux-rbs</td>
<td width="300">plux-rbs</td>
<td width="200">-<td>
<td width="200">-<td>
 +
<td width="300">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
<td width="200">-<td>
-
<td width="200">-<td>
+
<td width="50">no<td>
-
<td width="200">-<td>
+
-
<td width="200">-<td>
+
-
<td width="350">-<td>
+
-
<td width="50">-<td>
+
<td width="50">no<td>
<td width="50">no<td>
</tr>
</tr>
Line 130: Line 85:
<td width="200">[http://partsregistry.org/Part:BBa_K247003 K247003]</td>
<td width="200">[http://partsregistry.org/Part:BBa_K247003 K247003]</td>
<td width="300">[http://partsregistry.org/Part:BBa_J13002 pTet+rbs]+[http://partsregistry.org/Part:BBa_K247001 LuxR Mutant(Dull)]</td>
<td width="300">[http://partsregistry.org/Part:BBa_J13002 pTet+rbs]+[http://partsregistry.org/Part:BBa_K247001 LuxR Mutant(Dull)]</td>
-
<td width="200">-<td>
 
<td width="200">pSB1A2<td>
<td width="200">pSB1A2<td>
-
<td width="200">pMB1<td>
+
<td width="300">-<td>
-
<td width="200">-<td>
+
<td width="200">-<td>
<td width="200">-<td>
<td width="200">Amp<td>
<td width="200">Amp<td>
-
<td width="350">Glycerol stock in -80/miniprepped<td>
+
<td width="50">not yet<td>
-
<td width="50">ok<td>
+
<td width="50">OK<td>
-
<td width="50">[[Image:Chiba check.png]]<td>
+
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/Part:K247004 K247004]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_K247004 K247004]</td>
<td width="300">[http://partsregistry.org/Part:BBa_K027002 pLux+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K247000 mRFP(no Nco1)]</td>
<td width="300">[http://partsregistry.org/Part:BBa_K027002 pLux+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K247000 mRFP(no Nco1)]</td>
-
<td width="200">-<td>
 
<td width="200">pSB3C5<td>
<td width="200">pSB3C5<td>
-
<td width="200">p15A<td>
+
<td width="300">-<td>
-
<td width="200">-<td>
+
<td width="200">-<td>
<td width="200">-<td>
<td width="200">Cm<td>
<td width="200">Cm<td>
-
<td width="350">miniprepped<td>
 
<td width="50">Not yet<td>
<td width="50">Not yet<td>
-
<td width="50">[[Image:Chiba check.png]]<td>
+
<td width="50">OK<td>
</tr>
</tr>

Latest revision as of 22:50, 21 October 2009

E.coli Time Manager


The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Parts Status(Parts created by Chiba)

Part Name Sub Parts Plasmid Vector Part Length(bp) Part and vector length(bp) Resistance Sequenced Sent
K247000 removed the enzyme site "Nco1" - - - - no no
K247001 mutant LuxR(Dull) - - - - no no
K247002 plux-rbs - - - - no no
K247003 pTet+rbs+LuxR Mutant(Dull) pSB1A2 - - Amp not yet OK
K247004 pLux+rbs+mRFP(no Nco1) pSB3C5 - - Cm Not yet OK