Team:Chiba/Parts

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<html><link rel="stylesheet" href="https://2009.igem.org/wiki/index.php?title=User:Yoshimi/chiba1.css&action=raw&ctype=text/css" type="text/css" /></html>
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:::::<Font Size="7">'''''E''.coli Time Manager '''</Font> <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
 
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This is a template page. READ THESE INSTRUCTIONS.
 
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
 
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Example_logo.png|200px|right|frame]]
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|-
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Chiba | Team Example]]
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Modeling|Modeling]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Links|Links]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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{| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Arial', sans-serif;"
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|- style="background:#BC8F8FF; height:100px;" valign="top"
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<table class="calendar" width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
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<tr><td>
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'''[[Team:Chiba/Project|The Project]]'''
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</td></tr><tr><td>
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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</td></tr><tr><td>
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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</td></tr><tr><td>
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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</td></tr><tr><td>
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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</td></tr><tr><td>
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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</td></tr><tr><td>
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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__NOTOC__
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==Parts Status(Parts created by Chiba)==
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<table width="100%" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
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<tr>
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<td width="200">Part Name</td>
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<td width="300">Sub Parts</td>
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<td width="200">Plasmid Vector<td>
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<td width="300">Part Length(bp)<td>
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<td width="200">Part and vector length(bp)<td>
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<td width="200">Resistance<td>
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<td width="50">Sequenced<td>
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<td width="50">Sent<td>
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===Note===
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</tr>
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<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K247000 K247000]</td>
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<td width="300">removed the enzyme site "Nco1"</td>
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<td width="200">-<td>
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<td width="300">-<td>
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<td width="200">-<td>
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<td width="200">-<td>
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<td width="50">no<td>
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<td width="50">no<td>
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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</tr>
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<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K247001 K247001]</td>
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<td width="300">mutant LuxR(Dull)</td>
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<td width="200">-<td>
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<td width="300">-<td>
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<td width="200">-<td>
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<td width="200">-<td>
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<td width="50">no<td>
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<td width="50">no<td>
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</tr>
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<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K247002 K247002]</td>
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<td width="300">plux-rbs</td>
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<td width="200">-<td>
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<td width="300">-<td>
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<td width="200">-<td>
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<td width="200">-<td>
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<td width="50">no<td>
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<td width="50">no<td>
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</tr>
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<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K247003 K247003]</td>
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<td width="300">[http://partsregistry.org/Part:BBa_J13002 pTet+rbs]+[http://partsregistry.org/Part:BBa_K247001 LuxR Mutant(Dull)]</td>
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<td width="200">pSB1A2<td>
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<td width="300">-<td>
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<td width="200">-<td>
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<td width="200">Amp<td>
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<td width="50">not yet<td>
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<td width="50">OK<td>
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</tr>
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<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K247004 K247004]</td>
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<td width="300">[http://partsregistry.org/Part:BBa_K027002 pLux+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K247000 mRFP(no Nco1)]</td>
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<td width="200">pSB3C5<td>
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<td width="300">-<td>
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<td width="200">-<td>
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<td width="200">Cm<td>
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<td width="50">Not yet<td>
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<td width="50">OK<td>
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</tr>
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</table>
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Latest revision as of 22:50, 21 October 2009

E.coli Time Manager


The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Parts Status(Parts created by Chiba)

Part Name Sub Parts Plasmid Vector Part Length(bp) Part and vector length(bp) Resistance Sequenced Sent
K247000 removed the enzyme site "Nco1" - - - - no no
K247001 mutant LuxR(Dull) - - - - no no
K247002 plux-rbs - - - - no no
K247003 pTet+rbs+LuxR Mutant(Dull) pSB1A2 - - Amp not yet OK
K247004 pLux+rbs+mRFP(no Nco1) pSB3C5 - - Cm Not yet OK