Team:Chiba/Project

From 2009.igem.org

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=== Constructing A Flow of Activation ===
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Revision as of 09:33, 2 September 2009

Home

The Project

1, Constructing A Delay Switch

2, Constructing A Flow of Activation

  • Parallel
  • Series


Constructing A Delay Switch

  • 去年の紹介


Parallel communication

  • Testing
  • Results


Series communication

  • Testing
  • Results


Contents

Introduction

Project Design

Signaling System

In this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. (more about quorum sensing)


Constructing A Delay Switch, Multiple Ways

2008のことを述べ、Mutantの実験をやってない(今年はやる)とせつめい。

In principle, there are three ways to delay the activation of chemical communications;

  1. Silencing the Speakers: Rate of signal accumulation down-regulated, for instance, by slowing down the signal generators.
  2. Desensitize Receivers: Switching threshold elevated, for instance, by using insensitive receiver/ reporter systems.
  3. Partial Blocking: Decreasing the by chewing the signal up.

Constructing A Flow of Activation

1, Parallel Communication

Team:Chiba/Project/Parallel


2, Series communication

Team:Chiba/Project/Series

Conclusions