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Team:Chiba/Project
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=== Signaling System === | === Signaling System === | ||
| - | + | In this project, we use acylated homoserine lactones (AHLs), signaling | |
| - | + | molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. | |
| + | '''Senders''' express LuxI or similar enzymes, which catalyze the production | ||
| + | of AHLs, under the control of a constitutive (Tet) promoter. Each cell | ||
| + | thus generates AHL more or less at a constant rate. AHL can freely | ||
| + | permeate cell membranes and are detected by neighboring cells. | ||
| + | '''Receivers''' constitutively express LuxR proteins (or a similar | ||
| + | ortholog), the protein that detects AHL concentrations. When AHLs bind | ||
| + | LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The | ||
| + | threshold [AHL] at which switching occurs is determined by the | ||
| + | affinity of AHL for the particulr LuxR ortholog.[[Team:Chiba/Reference|<sup>(3),(4)</sup>]]. | ||
| + | [[Team:Chiba/Project/Quorum_Sensing|(more about quorum sensing)]] | ||
=== 1, parallel communication === | === 1, parallel communication === | ||
Revision as of 07:55, 2 September 2009
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AbstractIntroductionProject DesignSignaling SystemIn this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog.(3),(4). (more about quorum sensing) 1, parallel communication2, Series communicationConclusions |
