Team:Chiba/Project

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Home

The Project

1, Parallel communication

-LuxR Mutants

2, Series communication

-Las Check

Modeling

Conclusions

Introduction

Project Design

Signaling System

In this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. (more about quorum sensing)

Constructing A Delay Switch, Multiple Ways

2008のことを述べ、Mutantの実験をやってない（今年はやる）とせつめい。

In principle, there are three ways to delay the activation of chemical communications;

Silencing the Speakers: Rate of signal accumulation down-regulated, for instance, by slowing down the signal generators.
Desensitize Receivers: Switching threshold elevated, for instance, by using insensitive receiver/ reporter systems.
Partial Blocking: Decreasing the by chewing the signal up.

@@ Line 60: / Line 60: @@
 ===Constructing A Delay Switch, Multiple Ways ===
-のことを述べる。
+のことを述べ、Mutantの実験をやってない（今年はやる）とせつめい。
 In principle, there are three ways to delay the activation of chemical communications;