Team:Chiba/Project/Signaling-system

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(New page: ==== Signaling System ==== *なんじゃこりゃーなおします。--Yoshimi 13:22, 20 October 2009 (UTC) *sender使わないからいらないのでは？というか...)
 
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:::::<Font Size="7">'''''E''.coli Time Manager '''</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Links|Links]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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<table class="calendar" width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
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'''[[Team:Chiba/Project|The Project]]'''
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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__NOTOC__
==== Signaling System ====
==== Signaling System ====
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*なんじゃこりゃーなおします。--[[User:Yoshimi|Yoshimi]] 13:22, 20 October 2009 (UTC)
 
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*sender使わないからいらないのでは？というか，cell-cell comunicationは使ってなくてただのtranscription activatorとしてluxRを使っているだけ...という面がつよい...ので，luxRの転写活性化がどのようにおこるか，をメインにしてはいかが?--[[User:Maiko|Maiko]] 13:43, 20 October 2009 (UTC)
 
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*次項のスクリーニングでsenderを使うので、ここでも図にSenderを入れておくべきだと考えます。--[[User:Yoshimi|Yoshimi]] 14:10, 20 October 2009 (UTC)
 
[[Image:Chiba_quorumsensing.gif|600px]]
[[Image:Chiba_quorumsensing.gif|600px]]

Latest revision as of 22:55, 21 October 2009

E.coli Time Manager


Home The Team Parts Reference Notebook Protocols Links Acknowledgements Contact

The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Signaling System

Chiba quorumsensing.gif


In this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. about quorum sensing)

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