Team:Chiba/Project/for painting

From 2009.igem.org

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The protocol of this experiment is
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%%*Discussion
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%%The best condition that 最もmutant LuxR間の光り始める時刻の差を確認しやすかった条件は、以下の通りであった。
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Reporter : GFPuv
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medium : agar 5%
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thickness : 7 mm
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We thought it could be この条件でデモをやれば、各mutant LuxRの個性（遅れ方の特徴）を最大限に活かすことができると考えた。
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そうするとメリハリのあるきれいなアニメーションを描くことが出来るのではないか。
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また、使ったReporterによって光り始める時刻に差があったのでこちらにまとめます。
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[[Team:Chiba/Project/reporter|Distinction of fluorescence protein expression between reporters]]
[[Team:Chiba/Project/reporter|Distinction of fluorescence protein expression between reporters]]

Latest revision as of 00:32, 22 October 2009

back

To draw more better picture

  • we screened the "agar media condition" (the thickness & the agar conc.) to enlarge difference in the delay of the LuxR mutant.


  • Finding more useful condition of medium to demonstrate
Chiba 10mL1%mts-GFPuv1625.jpg
Chiba 40mL1%mts-GFPuv1625.jpg
Chiba 10mL5%mts-GFPuv1625.jpg
Chiba 40mL5%mts-GFPuv1625.jpg


We researched the best condition of medium to display our demonstration more clearly.

The protocol of this experiment is here.


  • About the difference of responce of each reporters

First, we did noted above experiment using GFPuv as reporter. Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.


As a result, we found that GFPuv is better reporter for our demonstration.


The protocol of this experiment is Distinction of fluorescence protein expression between reporters

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