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Team:Chiba/Project:Sandbox
From 2009.igem.org
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Contents |
Chiba 2009 Project: A Bacterial Timer
Introduction
- our project is to make a "bacterial timer" i.e. ~~.
- our approach to this goal is to make a series of a transcription factor which each of them differes in a responce time(?) of the transcription activation(?) by an single(same?) inducer.
- we believe that this device would be useful for making an macroscopic(?) timing control(?) in bacterial behavior or many application in synthetic biology & iGEM community.
- to demonstrate this "timing control", we aimed(?) to draw an "animated picture": a picture that pop up (emerge?) gradually!!
Project Design
- We chose an trascription factor LuxR (to make a series of ~~...)
- LuxR is from Vibrio fischeri: this protein is used in Quorum Seinsing. when LuxR binds to N-acyl homoserine lactone (AHL), LuxR-AHL complex is dimerilized and then activate the transcription below(?) the lux box promoter(?).
- we used the directed evolution method to make an series of LuxR
