Team:Chiba/Reference

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E.coli Time Manager


The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Reference

  1. Michiko E. Taga. Bonnie L.Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,100.suppl.2
  2. Melissa B. Miller and Bonnie L. Bassler.:QUORUM SENSING IN BACTERIA.annurev.micro.55.1.165.2001
  3. A. L. SCHAEFER et al.:Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry
  4. C. H. Collins.et al.:Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.55(3).712–723
  5. Raychaudhuri.A et al.:Chemical Mechanism and Substrate Specificity of RhlI, an Acylhomoserine Lactone Synthase from Pseudomonas aeruginosa.Biochemistry,44(8),2974 -2981,2005
  6. J P Pearson.et al.:Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,91,No. 1 ,197-201
  7. M K Winson et al.:Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),92No.20,9427-9431
  8. M.K Winson et al.:Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192
  9. D. J. Sayut et al.:Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.363(3),2007,667-673.