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Team:CityColSanFrancisco/Material
From 2009.igem.org
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'''Genetic manipulation ''' | '''Genetic manipulation ''' | ||
| - | *'''P. aeruginosa- source of OprB gene (membrane porin sequence).''' | + | *'''P. aeruginosa- source of OprB gene[https://static.igem.org/mediawiki/2009/8/82/P.aeruginosaOprBgene.txt] (membrane porin sequence).''' |
*'''pJQ200SK plasmid suicide vector for R. palustris. ''' | *'''pJQ200SK plasmid suicide vector for R. palustris. ''' | ||
*'''E. coli- for plasmid production/ purification. ''' | *'''E. coli- for plasmid production/ purification. ''' | ||
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== '''Primers for isolating and manipulating genes''' == | == '''Primers for isolating and manipulating genes''' == | ||
Revision as of 03:49, 22 October 2009
Bacteria
Chemotropes which reduce Fe3:
- S. Oneidensis
- R. ferireducines
Phototropes which Oxidize Fe2+
- R. palustris – hopefully transformed!
Genetic manipulation
- P. aeruginosa- source of OprB gene[1] (membrane porin sequence).
- pJQ200SK plasmid suicide vector for R. palustris.
- E. coli- for plasmid production/ purification.
Primers for isolating and manipulating genes
Pseudomonas aeruginosa OprB primer
Forward: 5' ATGTACAAGAACAAGAAAACC 3' Reverse: 5' TCAGAACACCGTCTGGATC 3'
Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick
Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3'
Battery
