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Team:CityColSanFrancisco/Material
From 2009.igem.org
Bacteria
Chemotropes which reduce Fe3:
- S. Oneidensis
- R. ferireducines
Phototropes which Oxidize Fe2+
- R. palustris TIE-1 strain – hopefully transformed!
Genetic manipulation
- P. aeruginosa- source of OprB gene[1] (membrane porin sequence).
- pJQ200SK plasmid suicide vector for R. palustris.
- E. coli- for plasmid production/ purification.
Primers for isolating and manipulating genes
Pseudomonas aeruginosa OprB primer
Forward: 5' ATGTACAAGAACAAGAAAACC 3' Reverse: 5' TCAGAACACCGTCTGGATC 3'
Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick
Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3'
R. palustris OprB primer -R. palustris might be upregulated
Forward: 5' ATGCTGCCGTCGCGCTGGCT 3' Reverse: 5' TCAGAACTGCGTGCGCAGTCG 3'
Battery
