Team:DTU Denmark

From 2009.igem.org

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<p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p>
<p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p>
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<font size="3"><b>The USER<sup>TM</sup> fusion primer design softwars</b></font><br>
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<a href="https://2009.igem.org/Team:DTU_Denmark/USERprogram" CLASS=leftbar>Design your primers here</a><br><br>

Revision as of 13:35, 17 October 2009

Wiki banner 967px.png

Welcome to the DTU iGEM wiki!


Sponsors and acknowledgements

- List of sponsors
- Acknowledgement

DTU Denmark


Project abstract

The Redoxilator

By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.

The redox coupled system

The USER fusion standard

Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.

The USERTM fusion primer design softwars
Design your primers here

NEWS NEWS NEWS NEWS


28.09.2009

The wiki page layout is now settled. Stay tuned for project results!



25.09.2009

We just received word that Lundbeckfonden has granted us $8000 USD for completion of our project! We owe them our gratitude, and continue our work delighted that thanks to Lundbeckfonden, Otto Mønsteds Fond, and Center for Microbial Biotechnology, we are now 100% funded and ready for iGEM 2009!



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