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Team:DTU Denmark/project
From 2009.igem.org
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<font size="3"><b>The USER fusion standard</b></font><br> | <font size="3"><b>The USER fusion standard</b></font><br> | ||
| - | <p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p> | + | <p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p> <br> |
<font size="3"><b>Biobricks designed and submitted</b></font><br> | <font size="3"><b>Biobricks designed and submitted</b></font><br> | ||
| - | <p align="justify"> | + | <p align="justify"> We have constructed and submitted 4 novel biobricks, specifically designed to be useful for construction of devices in the future: <br> |
| + | 1) A GFP variant optimized for expression in yeast <br> | ||
| + | 2) A protein destabilization sequence, which allows rapid protein turnover when appended to any protein <br> | ||
| + | 3) A device made from 1+2 as a proof of concept and very useful biobrick in itself: A fast degradable GFP that has a halflife of 30 min. compared to 7 hours without destabilization. <br> | ||
| + | 4) A USER(TM) cassette that will allow insertion of PCR fragments using the novel USER(TM) biobrick assembly standard. <br> | ||
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| + | More details about our biobricks under "parts submitted" | ||
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| + | </p> | ||
</font> | </font> | ||
Revision as of 10:43, 17 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
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The redoxilator - Theoretical background - Yeast as a model organism - Practical approach The USER assembly standard - Principle - Proof of concept - Manual - Primer design software |
The project Project abstract The Redoxilator By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast. 
The USER fusion standard Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions. Biobricks designed and submitted We have constructed and submitted 4 novel biobricks, specifically designed to be useful for construction of devices in the future: |
Synthetic Biology “Synthetic Biology is an art of engineering new biological systems that don’t exist in nature.” -Paras Chopra & Akhil Kamma In nature, biological molecules work together in complex systems to serve purposes of the cell. In synthetic biology these molecules are used as individual functional units that are combined to form tailored systems exhibiting complex dynamical behaviour. From ‘design specifications’ generated from computational modelling, engineering-based approaches enables the construction of such new specified gene-regulatory networks. The ultimate goal of synthetic biology is to construct systems that gain new functions, and the perspectives of the technology are enormous. It has already been used in several medical projects2 and is predicted to play a major role in biotech-production and environmental aspects. |
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