Team:EPF-Lausanne

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==Project Abstract==
==Project Abstract==
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Recent discoveries of photoreceptors in many organisms gave us insights into a possible interest of using light responsive genetic tools in synthetic biology.  
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Recent discoveries of photoreceptors in many organisms have given us insights into the interest of using light-responsive genetic tools in synthetic biology. The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus.
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The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus.  
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We will use light-sensitive DNA binding proteins (or light-sensitive proteins that activate DNA binding proteins) to convert a light input into a chosen output, for example fluorescence, through a reporter gene such as RFP.
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Demonstrating that the light-induced gene switch tool works in vivo would show that easier and faster tools can potentially be made available in several fields of biology, as such tools can induce more localized, more precise (time resolution and reversibility) and drastically faster genetic changes than currently used ones, thus allowing research to evolve even better.
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We will use light sensitive DNA binding proteins, or light sensitive proteins that activate DNA binding proteins to transduce a light input into a chosen output, for example reporter genes like GFP or RFP.
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The genetic circuits allowing us to measure the activity and responsiveness of light sensitive proteins are already designed, whereas the parts and biobricks required to engineer these circuits are still in formation.
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If we demonstrate that the light-induced-gene switch tool works in vivo, it would show that easier and faster tools could be used in several fields of biology. It would induce more localized, more precise (time resolution and reversibility) and drastically faster genetic changes than the current used tools, which will then allow research to evolve even better.
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LOV means light, oxygen, and voltage, whereas TAP means tryptophan-activated protein.
LOV means light, oxygen, and voltage, whereas TAP means tryptophan-activated protein.

Revision as of 09:54, 21 September 2009

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link "www.ubs.com"



Project Abstract

Recent discoveries of photoreceptors in many organisms have given us insights into the interest of using light-responsive genetic tools in synthetic biology. The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus. We will use light-sensitive DNA binding proteins (or light-sensitive proteins that activate DNA binding proteins) to convert a light input into a chosen output, for example fluorescence, through a reporter gene such as RFP. Demonstrating that the light-induced gene switch tool works in vivo would show that easier and faster tools can potentially be made available in several fields of biology, as such tools can induce more localized, more precise (time resolution and reversibility) and drastically faster genetic changes than currently used ones, thus allowing research to evolve even better. LOV means light, oxygen, and voltage, whereas TAP means tryptophan-activated protein.


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