Team:EPF-Lausanne/Modeling

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Modeling overview



Protein domain of interest

Our protein of interest is LOVTAP. This protein was sythetically engineered by Sosnick group. It is a fusion protein between a LOV domain (Avena Sativa phototropin 1) and the E. Coli tryptophan repressor. This protein undergoes changes under light activation as shown by Sosnick et al, in fact when the protein is activated by light it binds DNA and inversely. For more information about LOVTAP protein please click here.

Goal

Starting material

Both LOV domain crystallography files were obtained from RCSB:

Light activated LOV domain
Dark LOV domain

These crystallographies were done by Halavaty et al..

Molecular dynamics: a little theory

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