From 2009.igem.org
Wet
GVP Cluster
Restriction for Assembly
The vector pSB1AC3 containing the high, medium and low constitutive promoters were cut with PstI and SpeI to create correct ends for insert of GVP biobrick BBa_I750016, which was cut with XbaI and PstI.
- 3 to 10μL plasmid in MQ (1.0μg)
- 6 to 13μL MQ (end volume of 20μL)
- 2μL Fast digest buffer
- 1μL PstI fast digest enzyme
- 1μL SpeI/XbaI fast digest enzyme
Restriction was kept at 37C for 30 min. and put on ice until used for gel purification.
Purification
Transporters
Metal Accumulation
Vectors
Dry