Team:Groningen/Notebook/12 August 2009
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===Metal Accumulation=== | ===Metal Accumulation=== | ||
+ | MBP-ArsR fusion protein ligation was transformed for a second try, plated out on LB-amp<sub>100</sub> | ||
+ | (50 μL and concentrated after centrifugation) | ||
+ | Positive control (pSB1AC3-high constitutive promotor) ∞ | ||
+ | Negative control (pSB1AC3-ccdb)1 colony on the concentrated plate | ||
+ | Ligations had no colonies. | ||
===Vectors=== | ===Vectors=== |
Revision as of 07:45, 12 August 2009
Wet
GVP Cluster
TODO isolate plasmids from o.n. cultures
TODO run gel to check plasmid size (should be ~9000bp)
Transporters
Metal Accumulation
MBP-ArsR fusion protein ligation was transformed for a second try, plated out on LB-amp100 (50 μL and concentrated after centrifugation) Positive control (pSB1AC3-high constitutive promotor) ∞ Negative control (pSB1AC3-ccdb)1 colony on the concentrated plate Ligations had no colonies.
Vectors
Dry
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