Team:Groningen/Notebook/14 July 2009
From 2009.igem.org
(Difference between revisions)
(→GVP Cluster) |
(→GVP Cluster) |
||
Line 4: | Line 4: | ||
===GVP Cluster=== | ===GVP Cluster=== | ||
+ | |||
+ | [[Image:Plasmid Miniprep Kit Flow Protocol.gif|thumb|200px| www.sigmaaldrich.com]] | ||
'''Plasmid Purification''' | '''Plasmid Purification''' |
Revision as of 09:06, 14 July 2009
Wet
GVP Cluster
Plasmid Purification
Plasmid isolation was performed on the cultures of GVP and Terminator containing cells with the "Sygma-Aldrich™ GenElute™ Plasmid Miniprep Kit".
- From each tube 5mL of culture was collected in a 2mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Cells were resuspended in 200μL Resuspension Solution by up and down pipetting.
- To the mixture 200μL Lysis Solution was added, mixed by inverting the cup, and stored at room temperature for 5 minutes.
- 350μL of Neutralisation Solution was added and the tubes inverted.
- Cell debri was pelleted by centrifugation at full speed for 1 min.
Concentration of Plasmids
The concentration of isolated plasmid was determined with the use of a nano-drop.
GVP eluted in MQ
- ng/μL
- (260/280)
- (260/230)
Transporters
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|