From 2009.igem.org
Wet
GVP Cluster
Plasmid isolation
Plasmid isolation was performed on the pellets of E.coli TOP10 plasmids (made on saterday and stored in -20box) with the "Sygma-Aldrich™ GenElute™ Plasmid Miniprep Kit".
- From each tube 5mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Plasmids were eluted with 30μL MQ and stored in the fridge
Concentrations
| Plasmid
| Conc. ng/μL
| 260/280
| 260/230
| -20 box (michael
| Restriction Control
|
| pSB2K3-pZntR-GVP (12L, no.1)
| x
| x
| x
| x
| Yes (EcoRI/PstI)
|
| pSB2K3-pZntR-GVP (12L, no.2)
| x
| x
| x
| x
| Yes (EcoRI/PstI)
|
| pSB2K3-pCueO-GVP (12L, no.1)
| x
| x
| x
| x
| Yes (EcoRI/PstI)
|
| pSB2K3-pCueO-GVP (12L, no.2)
| x
| x
| x
| x
| Yes (EcoRI/PstI)
|
Transporters
Metal Accumulation
Vectors
Dry
"
