Team:Groningen/Notebook/15 July 2009
From 2009.igem.org
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'''Restriction for Assembly''' | '''Restriction for Assembly''' | ||
- | The vector containing GVP cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector. | + | The vector ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035) containing [http://partsregistry.org/wiki/index.php/Part:BBa_I750016 GVP] cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002]). |
* 10μL MQ | * 10μL MQ |
Revision as of 07:44, 15 July 2009
Wet
GVP Cluster
Restriction for Assembly
The vector (BBa_J61035) containing [http://partsregistry.org/wiki/index.php/Part:BBa_I750016 GVP cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector (BBa_J61002).
- 10μL MQ
- 6μL plasmid in MQ (2μg)
- 2μL Fast digest buffer
- 1μL PstI fast digest enzyme
- 1μL XbaI fast digest enzyme
The vector containing promotor BBa_J23100 was cut with SpeI and PstI.
- 4μL MQ
- 10μL plasmid in MQ
- 2μL Fast digest buffer
- 1μL SpeI fast digest enzyme
- 1μL PstI fast digest enzyme
Gel electroforesis
24μL of each sample was loaded on a 1% agarose gel with EtBr and a 1kb ladder was used (see picture).
- → From left to right: 1kb Marker,
Transporters
Dry
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