Team:Groningen/Notebook/15 July 2009
From 2009.igem.org
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* 1μL SpeI fast digest enzyme | * 1μL SpeI fast digest enzyme | ||
* 1μL PstI fast digest enzyme | * 1μL PstI fast digest enzyme | ||
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+ | '''Gel electroforesis''' | ||
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+ | 24μL of each sample was loaded on a 1% agarose gel with EtBr and a 1kb ladder was used (see picture). | ||
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+ | [[Image:]] [[Image:Generulers_1kb_marker_Fermentas.jpg]] | ||
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+ | :→ From left to right: 1kb Marker, | ||
===Transporters=== | ===Transporters=== |
Revision as of 07:38, 15 July 2009
Wet
GVP Cluster
Restriction for Assembly
The vector containing GVP cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector.
- 10μL MQ
- 6μL plasmid in MQ (2μg)
- 2μL Fast digest buffer
- 1μL PstI fast digest enzyme
- 1μL XbaI fast digest enzyme
The vector containing promotor BBa_J23100 was cut with SpeI and PstI.
- 4μL MQ
- 10μL plasmid in MQ
- 2μL Fast digest buffer
- 1μL SpeI fast digest enzyme
- 1μL PstI fast digest enzyme
Gel electroforesis
24μL of each sample was loaded on a 1% agarose gel with EtBr and a 1kb ladder was used (see picture).
- → From left to right: 1kb Marker,
Transporters
Dry
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