Team:Groningen/Notebook/15 July 2009

From 2009.igem.org

(Difference between revisions)
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m (GVP Cluster)
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* 1μL  SpeI fast digest enzyme
* 1μL  SpeI fast digest enzyme
* 1μL  PstI fast digest enzyme
* 1μL  PstI fast digest enzyme
 +
 +
'''Gel electroforesis'''
 +
 +
24μL of each sample was loaded on a 1% agarose gel with EtBr and a 1kb ladder was used (see picture).
 +
 +
[[Image:]] [[Image:Generulers_1kb_marker_Fermentas.jpg]]
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 +
:→ From left to right: 1kb Marker,
===Transporters===
===Transporters===

Revision as of 07:38, 15 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Restriction for Assembly

The vector containing GVP cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector.

  • 10μL MQ
  • 6μL plasmid in MQ (2μg)
  • 2μL Fast digest buffer
  • 1μL PstI fast digest enzyme
  • 1μL XbaI fast digest enzyme

The vector containing promotor BBa_J23100 was cut with SpeI and PstI.

  • 4μL MQ
  • 10μL plasmid in MQ
  • 2μL Fast digest buffer
  • 1μL SpeI fast digest enzyme
  • 1μL PstI fast digest enzyme

Gel electroforesis

24μL of each sample was loaded on a 1% agarose gel with EtBr and a 1kb ladder was used (see picture).

[[Image:]] Generulers 1kb marker Fermentas.jpg

→ From left to right: 1kb Marker,

Transporters

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30