Team:Groningen/Notebook/15 September 2009

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Wet

GVP Cluster

Planning

TODO work out the wiki page for GVP
TODO make a doodle for presentation planning (1-19 oct.)
TODO media attention
TODO


Plates

The

Name Plasmid Used Antibiotics on Plasmid No. of Colonies Date
GlpF no.1 (low) pSB1AC3 Ampicillin 0 14 sept.
GlpF no.1 (high) pSB1AC3 Ampicillin 2 14 sept.
GlpF no.2 (low) pSB1AC3 Ampicillin 1 14 sept.
GlpF no.2 (high) pSB1AC3 Ampicillin 1 14 sept.
pLacI (low) pSB1AC3 Ampicillin ~30 14 sept.
pLacI (high) pSB1AC3 Ampicillin ~80 14 sept.
pLacI-GVP no.1 (low) pSB1A2 Ampicillin 0 14 sept.
pLacI-GVP no.1 (high) pSB1A2 Ampicillin 4 14 sept.
pLacI-GVP no.2 (low) pSB1A2 Ampicillin 0 14 sept.
pLacI-GVP no.2 (high) pSB1A2 Ampicillin 9 14 sept.
Positive (J23101) (high) J61002 Ampicillin ~1500 14 sept.
Negative (MQ) (high) MQ Ampicillin 0 14 sept.


→ The plates showed a low amount of colonies, and also red in the case of the positive plate.


→ The two plates with stripes of pZntR-GVP (pSB2K3) and pCueO-GVP (pSB2K3) culture for glycerol stocks showed growth and some single colonies.


→ All plates were stored in the fridge for further use.


Cultures

The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30