From 2009.igem.org
Wet
GVP Cluster
Transporters
HmtA curios about the dirty experiment. a 1% gel control will show if expected product is there. Unfortunately there was no product, the primer dimer changed and pcr 1 and 2 remain available. PCR again. Different program.
Plus starting an other PCR1 and PCR2 to get new megaprimers.
Metal Accumulation
Restriction analysis MBP-ArsR
1% Agarose (1xTBE) gel with restriction analysis of MBP-ArsR fusion protein plasmids, with selfcloser (1), sample (white colony) (2), sample (red colony??) (3). Expected sizes of positive construct are shown. Samples were digested using PstI & XbaI, ~45 min 37 °C.
Sample MBP-ArsR (200) was isolated from red organims, indicating the presence of RFP or similar, this was however not engineered in this plasmid. Therefore a deviant restriction pattern was to be expected. It appears as if two different plasmids were present in this sample.
Vectors
Restriction analysis metal promotors
Dry