http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_June_2009&feed=atom&action=historyTeam:Groningen/Notebook/19 June 2009 - Revision history2024-03-28T11:38:15ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_June_2009&diff=12639&oldid=prevVerhoeven1981 at 11:26, 26 June 20092009-06-26T11:26:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*For the other plasmids several bands were seen.}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*For the other plasmids several bands were seen.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:Restriction19juni.jpeg|300px|thumb|right]]</ins></div></td></tr>
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</table>Verhoeven1981http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_June_2009&diff=11419&oldid=prevNienke at 15:43, 23 June 20092009-06-23T15:43:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*DNA was isolated from them, using the Nucleospin plasmid isolation kit.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*DNA was isolated from them, using the Nucleospin plasmid isolation kit.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Concentration of the samples were:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Concentration of the samples were:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">***??</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">***??</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">***??</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*For the other plasmids several bands were seen.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*For the other plasmids several bands were seen.}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Nienkehttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_June_2009&diff=10851&oldid=prevNienke: New page: {{Team:Groningen/Notebook/Day| *All o/n cultures were succesfull. *30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, ...2009-06-22T14:58:20Z<p>New page: {{Team:Groningen/Notebook/Day| *All o/n cultures were succesfull. *30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, ...</p>
<p><b>New page</b></p><div>{{Team:Groningen/Notebook/Day|<br />
*All o/n cultures were succesfull.<br />
*30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, gene nr1-3 and date)<br />
*DNA was isolated from them, using the Nucleospin plasmid isolation kit.<br />
**Concentration of the samples were:<br />
***??<br />
***??<br />
***??<br />
*1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.<br />
*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.<br />
*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.<br />
*For the other plasmids several bands were seen.<br />
}}</div>Nienke