Team:Groningen/Notebook/21 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== '''Planning''' :→ {{todo}} work out the wiki page for GVP :→ {{done}} make a doodle for presentation planning (1-1...) |
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===Transporters=== | ===Transporters=== | ||
+ | '''HmtA''' | ||
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+ | Nieuwe poging om HmtA te krijgen. Via verschillende routes, PCR1 met F1 mut1rc en EcoRI cut pBAD met 742 f2 en PCR1 & 2. | ||
===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 11:21, 21 September 2009
Wet
GVP Cluster
Planning
- → TODO work out the wiki page for GVP
- → DONE make a doodle for presentation planning (1-19 oct.)
- → TODO media attention
- → DONE place an ethics survey link on twitter
- → TODO clone pArsR-GVP into pSB2K3
- → TODO clone repeat out of GVP cluster
- → TODO make glycerol stocks of constructs
- → TODO enter info on part registry
Restriction for Assembly
The plasmid of pMA-gvpL insert was cut with MvaI/XhoI to cut out the entire part of wanted fragment.
Plasmid | Amount μL | MQ μL | R-digest buffer | MvaI digest enzyme | XhoI digest enzyme |
pArsR-GVP no.1 | 10.0 | 6.0 | 3.0 | 1.0 | 1.0 |
Transporters
HmtA
Nieuwe poging om HmtA te krijgen. Via verschillende routes, PCR1 met F1 mut1rc en EcoRI cut pBAD met 742 f2 en PCR1 & 2.
Metal Accumulation
Vectors
Dry
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