From 2009.igem.org
Wet
GVP Cluster
Planning
- → TODO work out the wiki page for GVP
- → DONE make a doodle for presentation planning (1-19 oct.)
- → TODO media attention
- → DONE place an ethics survey link on twitter
- → TODO clone pArsR-GVP into pSB2K3
- → TODO clone repeat out of GVP cluster
- → TODO make glycerol stocks of constructs
- → TODO enter info on part registry
Restriction for Assembly
The plasmid of pMA-gvpL insert was cut with MvaI/XhoI to cut out the entire part of wanted fragment.
Plasmid
| Amount μL
| MQ μL
| R-digest buffer
| MvaI digest enzyme
| XhoI digest enzyme
|
pArsR-GVP no.1
| 10.0
| 6.0
| 3.0
| 1.0
| 1.0
|
Transporters
HmtA
Nieuwe poging om HmtA te krijgen. Via verschillende routes, PCR1 met F1 mut1rc en EcoRI cut pBAD met 742 f2 en PCR1 & 2.
Metal Accumulation
Vectors
Dry