Team:Groningen/Notebook/22 August 2009
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(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== :→ {{todo}} Isolate plasmids from E.coli Top10 BBa_J23101 cells :→ {{todo}} Plate E.coli Top10 BBa_J23101 cells from...) |
(→GVP Cluster) |
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===GVP Cluster=== | ===GVP Cluster=== | ||
- | :→ {{ | + | :→ {{done}} Isolate plasmids from E.coli Top10 BBa_J23101 cells |
- | :→ {{ | + | :→ {{done}} Plate E.coli Top10 BBa_J23101 cells from o.n. culture |
- | :→ {{todo}} Pellet remaining cells for short storage in -20 box | + | :→ {{todo}} <strike>Pellet remaining cells for short storage in -20 box</strike> (not needed) |
- | :→ {{ | + | :→ {{done}} Place the four test cultures with and without GVP on table at room temperature (make foto) |
+ | |||
+ | :→ {{todo}} Look at plates stored at 37C with BBa_J61002-pArsR<sup>+</sup>/pZntR<sup>+</sup>/pCueO<sup>+</sup> and use colonies to grow o.n. cultures for plasmid isolation | ||
===Transporters=== | ===Transporters=== |
Revision as of 08:54, 22 August 2009
Wet
GVP Cluster
- → DONE Isolate plasmids from E.coli Top10 BBa_J23101 cells
- → DONE Plate E.coli Top10 BBa_J23101 cells from o.n. culture
- → TODO
Pellet remaining cells for short storage in -20 box(not needed)
- → DONE Place the four test cultures with and without GVP on table at room temperature (make foto)
- → TODO Look at plates stored at 37C with BBa_J61002-pArsR+/pZntR+/pCueO+ and use colonies to grow o.n. cultures for plasmid isolation
Transporters
Metal Accumulation
Vectors
Dry
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