Team:Groningen/Notebook/22 August 2009

(Difference between revisions)

Revision as of 08:54, 22 August 2009

→ DONE Isolate plasmids from E.coli Top10 BBa_J23101 cells

→ DONE Plate E.coli Top10 BBa_J23101 cells from o.n. culture

→ TODO ~~Pellet remaining cells for short storage in -20 box~~ (not needed)

→ DONE Place the four test cultures with and without GVP on table at room temperature (make foto)

→ TODO Look at plates stored at 37C with BBa_J61002-pArsR⁺/pZntR⁺/pCueO⁺ and use colonies to grow o.n. cultures for plasmid isolation

@@ Line 5: / Line 5: @@
 ===GVP Cluster===
-:→ {{todo}} Isolate plasmids from E.coli Top10 BBa_J23101 cells
+:→ {{done}} Isolate plasmids from E.coli Top10 BBa_J23101 cells
-:→ {{todo}} Plate E.coli Top10 BBa_J23101 cells from o.n. culture
+:→ {{done}} Plate E.coli Top10 BBa_J23101 cells from o.n. culture
-:→ {{todo}} Pellet remaining cells for short storage in -20 box
+:→ {{todo}} <strike>Pellet remaining cells for short storage in -20 box</strike> (not needed)
-:→ {{todo}} Place the four test cultures with and without GVP on table at room temperature (make foto)
+:→ {{done}} Place the four test cultures with and without GVP on table at room temperature (make foto)
+:→ {{todo}} Look at plates stored at 37C with BBa_J61002-pArsR<sup>+</sup>/pZntR<sup>+</sup>/pCueO<sup>+</sup> and use colonies to grow o.n. cultures for plasmid isolation
 ===Transporters===