Team:Groningen/Notebook/24 August 2009
From 2009.igem.org
(Difference between revisions)
m (→GVP Cluster) |
(→Vectors) |
||
Line 19: | Line 19: | ||
===Vectors=== | ===Vectors=== | ||
+ | ====Metal promotors==== | ||
+ | Protocol #3 | ||
+ | * Phosphorylation and annealing synthetic oligo's | ||
+ | * Ligation in pSB1AC3 | ||
==Dry== | ==Dry== | ||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} |
Revision as of 07:03, 24 August 2009
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
Vectors
Metal promotors
Protocol #3
- Phosphorylation and annealing synthetic oligo's
- Ligation in pSB1AC3
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|