Team:Groningen/Notebook/24 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== :→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid :→ {{todo}} ...) |
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===GVP Cluster=== | ===GVP Cluster=== | ||
+ | :→ {{todo}} Restriction of GVP and pSB1AC3-Lac/pBAD for assembly | ||
+ | :→ {{todo}} Gel purification of wanted fragments and nanodrop | ||
:→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid | :→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid | ||
:→ {{todo}} Send plasmids with ME<sup>tal</sup> promoters in BBa_J61002 vector for sequencing | :→ {{todo}} Send plasmids with ME<sup>tal</sup> promoters in BBa_J61002 vector for sequencing |
Revision as of 22:30, 22 August 2009
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
Vectors
Dry
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