Team:Groningen/Notebook/25 August 2009
From 2009.igem.org
(Difference between revisions)
(→GVP Cluster) |
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:→ {{todo}} Gel purification of wanted fragments and nanodrop | :→ {{todo}} Gel purification of wanted fragments and nanodrop | ||
:→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid | :→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid | ||
- | :→ {{ | + | :→ {{done}} Send plasmids with ME<sup>tal</sup> promoters in BBa_J61002 vector for sequencing |
- | :→ {{ | + | :→ {{done}} Check ME<sup>tal</sup> + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter) |
:→ {{todo}} Test promoter strenght compared to BBa_J23101 promoter (Sven) | :→ {{todo}} Test promoter strenght compared to BBa_J23101 promoter (Sven) | ||
- | :→ {{ | + | :→ {{done}} Enter sequences of constructs to Sandbox |
===Transporters=== | ===Transporters=== |
Revision as of 11:44, 25 August 2009
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → DONE Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → DONE Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → DONE Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
Vectors
Metal promotors
Cloning into pSB1AC3 and pSB3K3 has stopped
Dry
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