Team:Groningen/Notebook/25 August 2009

From 2009.igem.org

(Difference between revisions)
m (Metal promotors)
(GVP Cluster)
Line 4: Line 4:
===GVP Cluster===
===GVP Cluster===
 +
 +
:→ {{todo}} Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
 +
:→ {{todo}} Gel purification of wanted fragments and nanodrop
 +
:→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
 +
:→ {{todo}} Send plasmids with ME<sup>tal</sup> promoters in BBa_J61002 vector for sequencing
 +
:→ {{todo}} Check ME<sup>tal</sup> + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
 +
 +
:→ {{todo}} Test promoter strenght compared to BBa_J23101 promoter (Sven)
 +
:→ {{todo}} Enter sequences of constructs to Sandbox
===Transporters===
===Transporters===

Revision as of 14:46, 24 August 2009

Igemhomelogo.png

Wet

GVP Cluster

TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
TODO Gel purification of wanted fragments and nanodrop
TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
TODO Enter sequences of constructs to Sandbox

Transporters

Metal Accumulation

Vectors

Metal promotors

Cloning into pSB1AC3 and pSB3K3 has stopped

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30