Team:Groningen/Notebook/28 August 2009
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(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== :→ {{todo}} ===Transporters=== ===Metal Accumulation=== ===Vectors=== ==Dry== {{Team:Groningen/Notebook/Day/Foot...) |
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===GVP Cluster=== | ===GVP Cluster=== | ||
- | :→ {{todo}} | + | :→ {{todo}} Isolate plasmids from o.n. cultures |
+ | :→ {{todo}} Restriction analysis of plasmids for correct ligation of GVP behind LAC/pBAD promoter | ||
+ | |||
+ | :→ {{todo}} Test control of bouyancy in Saline solution | ||
+ | :→ {{todo}} Order synthetic DNA for GVP | ||
+ | |||
+ | |||
+ | '''Overnight Precultures''' | ||
+ | |||
+ | All tubes showed growth of E.coli cells, and must have ampicillin resistance on a plasmid(The tubes differed in cell density though). To be sure of correct ligation products in the plasmids an isolation will be performed, followed by restriction analysis. If positive, the plasmids will be sent for sequencing. | ||
+ | |||
+ | '''Overnight Plates''' | ||
===Transporters=== | ===Transporters=== |
Revision as of 08:11, 28 August 2009
Wet
GVP Cluster
- → TODO Isolate plasmids from o.n. cultures
- → TODO Restriction analysis of plasmids for correct ligation of GVP behind LAC/pBAD promoter
- → TODO Test control of bouyancy in Saline solution
- → TODO Order synthetic DNA for GVP
Overnight Precultures
All tubes showed growth of E.coli cells, and must have ampicillin resistance on a plasmid(The tubes differed in cell density though). To be sure of correct ligation products in the plasmids an isolation will be performed, followed by restriction analysis. If positive, the plasmids will be sent for sequencing.
Overnight Plates
Transporters
Metal Accumulation
Vectors
Dry
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