From 2009.igem.org
Wet
GVP Cluster
- → TODO Isolate plasmids from o.n. cultures
- → TODO Restriction analysis of plasmids for correct ligation of GVP behind LAC/pBAD promoter
- → TODO Test control of bouyancy in Saline solution
- → TODO Order synthetic DNA for GVP
Overnight Precultures
All tubes showed growth of E.coli cells, and must have ampicillin resistance on a plasmid(The tubes differed in cell density though). To be sure of correct ligation products in the plasmids an isolation will be performed, followed by restriction analysis. If positive, the plasmids will be sent for sequencing.
Overnight Plates
Transporters
Metal Accumulation
Vectors
Dry