Team:Groningen/Notebook/28 September 2009

From 2009.igem.org

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(GVP Cluster)
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::* Is it one of the restriction enzymes (SpeI/PstI) failing work
::* Is it one of the restriction enzymes (SpeI/PstI) failing work
::* Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)
::* Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)
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[[Image:28-9 no.1.jpg|340px]] [[Image:Generulers_1kb_marker_Fermentas.jpg]]
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:→ From left to right: 1kB ladder, Sample Steven, SpeI/PstI control with BBa_K190017, Empty Slot, GVP (6x)
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:→ For GVP two fragments of ~6000bp and ~3000bp are expected, and for BBa_K190017 fragments of ~900bp and ~2000bp are expected.
===Transporters===
===Transporters===

Revision as of 08:20, 28 September 2009

Igemhomelogo.png

Wet

GVP Cluster

TODO Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment

TODO Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments

TODO Ligate the three fragments into the vector and transform E.coli DB3 cells

TODO Find out wath the problem is with RBS??

  • Is it one of the restriction enzymes (SpeI/PstI) failing work
  • Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)


340px Generulers 1kb marker Fermentas.jpg

→ From left to right: 1kB ladder, Sample Steven, SpeI/PstI control with BBa_K190017, Empty Slot, GVP (6x)
→ For GVP two fragments of ~6000bp and ~3000bp are expected, and for BBa_K190017 fragments of ~900bp and ~2000bp are expected.

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30