Team:Groningen/Notebook/28 September 2009
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::* Is it one of the restriction enzymes (SpeI/PstI) failing work | ::* Is it one of the restriction enzymes (SpeI/PstI) failing work | ||
::* Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly) | ::* Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly) | ||
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+ | [[Image:28-9 no.1.jpg|340px]] [[Image:Generulers_1kb_marker_Fermentas.jpg]] | ||
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+ | :→ From left to right: 1kB ladder, Sample Steven, SpeI/PstI control with BBa_K190017, Empty Slot, GVP (6x) | ||
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+ | :→ For GVP two fragments of ~6000bp and ~3000bp are expected, and for BBa_K190017 fragments of ~900bp and ~2000bp are expected. | ||
===Transporters=== | ===Transporters=== |
Revision as of 08:20, 28 September 2009
Wet
GVP Cluster
TODO Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment
TODO Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments
TODO Ligate the three fragments into the vector and transform E.coli DB3 cells
TODO Find out wath the problem is with RBS??
- Is it one of the restriction enzymes (SpeI/PstI) failing work
- Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)
- → From left to right: 1kB ladder, Sample Steven, SpeI/PstI control with BBa_K190017, Empty Slot, GVP (6x)
- → For GVP two fragments of ~6000bp and ~3000bp are expected, and for BBa_K190017 fragments of ~900bp and ~2000bp are expected.
Transporters
Metal Accumulation
Vectors
Dry
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