Team:Groningen/Notebook/28 September 2009
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(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== {{todo}} Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment {{todo}} Restict GVP fragment and ...) |
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{{todo}} Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment | {{todo}} Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment | ||
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{{todo}} Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments | {{todo}} Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments | ||
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{{todo}} Ligate the three fragments into the vector and transform E.coli DB3 cells | {{todo}} Ligate the three fragments into the vector and transform E.coli DB3 cells | ||
Revision as of 07:41, 28 September 2009
Wet
GVP Cluster
TODO Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment
TODO Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments
TODO Ligate the three fragments into the vector and transform E.coli DB3 cells
TODO Find out wath the problem is with RBS??
- Is it one of the restriction enzymes (SpeI/PstI) failing work
- Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)
Transporters
Metal Accumulation
Vectors
Dry
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