Team:Groningen/Notebook/3 August 2009
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===Transporters=== | ===Transporters=== | ||
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===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 14:22, 3 August 2009
Wet
GVP Cluster
Transporters
DONE GlpF → pick colonies and use for overnight culture. (2 colonies in 6 mL LB-amp medium)
Metal Accumulation
TODO MBP
- Plasmid isolation (cells can be found in the freezer Jolanda)
- PCR of MBP
- PCR Mix
12,5 uL | Phusion 2x MM |
1 uL | MBP Fw |
1 uL | MBP rev |
0.5uL | MBP plasmid |
Vectors
Dry
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