Team:Groningen/Notebook/3 August 2009

From 2009.igem.org

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===GVP Cluster===
===GVP Cluster===
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'''Over Night Cultures'''
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Over night cultures in 4mL LB-amp<sub>100</sub> or LB-kan<sub>50</sub> medium were prepared from the following glycerol stocks:
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:→ E.coli TOP10 pSB3K3-high const. promoter (kan.)
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:→ E.coli TOP10 pSB3K3-med. const. promoter (kan.)
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:→ E.coli TOP10 pSB3K3-low const. promoter (kan.)
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 +
:→ E.coli TOP10 pSB1AC3-high const. promoter (amp.)
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:→ E.coli TOP10 pSB1AC3-med. const. promoter (amp.)
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:→ E.coli TOP10 pSB1AC3-low const. promoter (amp.)
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and put in the 37°C waterbath at 200 rpm.
===Transporters===
===Transporters===

Revision as of 14:31, 3 August 2009

Igemhomelogo.png

Wet

GVP Cluster

Over Night Cultures

Over night cultures in 4mL LB-amp100 or LB-kan50 medium were prepared from the following glycerol stocks:

→ E.coli TOP10 pSB3K3-high const. promoter (kan.)
→ E.coli TOP10 pSB3K3-med. const. promoter (kan.)
→ E.coli TOP10 pSB3K3-low const. promoter (kan.)
→ E.coli TOP10 pSB1AC3-high const. promoter (amp.)
→ E.coli TOP10 pSB1AC3-med. const. promoter (amp.)
→ E.coli TOP10 pSB1AC3-low const. promoter (amp.)

and put in the 37°C waterbath at 200 rpm.

Transporters

DONE GlpF → pick colonies and use for overnight culture. (2 colonies in 6 mL LB-amp medium)

Metal Accumulation

TODO MBP

  • Plasmid isolation (cells can be found in the freezer Jolanda)
  • PCR of MBP
  • PCR Mix
12,5 uL Phusion 2x MM
1 uL MBP Fw
1 uL MBP rev
0.5uL MBP plasmid

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30