Team:Groningen/Notebook/4 August 2009
From 2009.igem.org
(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation...) |
m (→GVP Cluster) |
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All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out... | All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out... | ||
+ | |||
+ | :→ {{todo}} isolate plasmids from precultures | ||
+ | :→ {{todo}} perform a restriction on the medium promoter containing plasmids with SpeI and PstI | ||
+ | :→ {{todo}} purify cut vectors with miniprep (11bp fragment should be lost) | ||
+ | :→ {{todo}} perform a ligation between cut vector and GVP fragment | ||
+ | :→ {{todo}} transform E.coli TOP10 competent cells with ligation product | ||
===Transporters=== | ===Transporters=== |
Revision as of 07:09, 4 August 2009
Wet
GVP Cluster
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
- → TODO isolate plasmids from precultures
- → TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
- → TODO purify cut vectors with miniprep (11bp fragment should be lost)
- → TODO perform a ligation between cut vector and GVP fragment
- → TODO transform E.coli TOP10 competent cells with ligation product
Transporters
Metal Accumulation
Vectors
Dry
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