Team:Groningen/Notebook/4 August 2009

From 2009.igem.org

(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation...)
m (GVP Cluster)
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All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
 +
 +
:→ {{todo}} isolate plasmids from precultures
 +
:→ {{todo}} perform a restriction on the medium promoter containing plasmids with SpeI and PstI
 +
:→ {{todo}} purify cut vectors with miniprep (11bp fragment should be lost)
 +
:→ {{todo}} perform a ligation between cut vector and GVP fragment
 +
:→ {{todo}} transform E.coli TOP10 competent cells with ligation product
===Transporters===
===Transporters===

Revision as of 07:09, 4 August 2009

Igemhomelogo.png

Wet

GVP Cluster

All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...

TODO isolate plasmids from precultures
TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
TODO purify cut vectors with miniprep (11bp fragment should be lost)
TODO perform a ligation between cut vector and GVP fragment
TODO transform E.coli TOP10 competent cells with ligation product

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30