Team:Groningen/Notebook/4 August 2009
From 2009.igem.org
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===Metal Accumulation=== | ===Metal Accumulation=== | ||
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+ | Restriction | ||
===Vectors=== | ===Vectors=== |
Revision as of 14:27, 4 August 2009
Wet
GVP Cluster
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
- → TODO isolate plasmids from precultures
- → TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
- → TODO purify cut vectors with miniprep (11bp fragment should be lost)
- → TODO perform a ligation between cut vector and GVP fragment
- → TODO transform E.coli TOP10 competent cells with ligation product
Transporters
GlpF
Restriction, ligation and transformation. Done as noted at 31 July .
Metal Accumulation
Fusion ArsR with MBP
Restriction
Vectors
Dry
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