Team:Groningen/Notebook/4 August 2009
From 2009.igem.org
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Restriction | Restriction | ||
+ | {| | ||
+ | | | ||
+ | {| | ||
+ | |10ul | ||
+ | |GlpF PCR | ||
+ | |- | ||
+ | |2 ul | ||
+ | |Buffer | ||
+ | |- | ||
+ | |05.ul | ||
+ | |EcoRI | ||
+ | |- | ||
+ | |0.5 ul | ||
+ | |SpeI | ||
+ | |- | ||
+ | |7 uL | ||
+ | |MQ | ||
+ | |- | ||
+ | |} | ||
+ | |width="10"| | ||
+ | | | ||
+ | <!--Tabel 2 hier--> | ||
+ | |width="10"| | ||
+ | | | ||
+ | <!--Tabel 3 hier--> | ||
+ | |} | ||
+ | : '''Restriction''' | ||
+ | {| | ||
===Vectors=== | ===Vectors=== |
Revision as of 14:29, 4 August 2009
Wet
GVP Cluster
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
- → TODO isolate plasmids from precultures
- → TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
- → TODO purify cut vectors with miniprep (11bp fragment should be lost)
- → TODO perform a ligation between cut vector and GVP fragment
- → TODO transform E.coli TOP10 competent cells with ligation product
Transporters
GlpF
Restriction, ligation and transformation. Done as noted at 31 July .
Metal Accumulation
Fusion ArsR with MBP
Restriction
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