From 2009.igem.org
Wet
GVP Cluster
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
- → TODO isolate plasmids from precultures
- → TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
- → TODO purify cut vectors with miniprep (11bp fragment should be lost)
- → TODO perform a ligation between cut vector and GVP fragment
- → TODO transform E.coli TOP10 competent cells with ligation product
Transporters
GlpF
Restriction, ligation and transformation. Done as noted at 31 July
.
Metal Accumulation
Fusion ArsR with MBP
Restriction
10ul
| GlpF PCR
|
2 ul
| Buffer
|
05.ul
| EcoRI
|
0.5 ul
| SpeI
|
7 uL
| MQ
|
|
|
|
|
|
- Restriction