http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&feed=atom&action=historyTeam:Groningen/Notebook/6 July 2009 - Revision history2024-03-29T08:08:13ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=19035&oldid=prevVerhoeven1981 at 10:13, 13 July 20092009-07-13T10:13:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Wet==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Wet==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===GVP Cluster===</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Transporters===</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Purification of HmtA (PA Q9I147 6xHis Tag)'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Purification of HmtA (PA Q9I147 6xHis Tag)'''</div></td></tr>
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</table>Verhoeven1981http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=17199&oldid=prevJolandaWitteveen: /* Wet */2009-07-09T12:19:58Z<p><span class="autocomment">Wet</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml <del class="diffchange diffchange-inline">LB</del>+amp</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml <ins class="diffchange diffchange-inline">TY</ins>+amp</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Incubate overnight 37°C with shaking</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Incubate overnight 37°C with shaking</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>JolandaWitteveenhttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=17171&oldid=prevVerhoeven1981: /* Wet */2009-07-09T11:48:36Z<p><span class="autocomment">Wet</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 11:48, 9 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* BBa_J23109 iGEM plate 2, 2G</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:</ins>BBa_J23109 <ins class="diffchange diffchange-inline">BBa_J23109] </ins>iGEM plate 2, 2G</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* BBa_J23100 iGEM plate 1, 18C</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:</ins>BBa_J23100 <ins class="diffchange diffchange-inline">BBa_J23100] </ins>iGEM plate 1, 18C</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* BBa_J23106 iGEM plate 1, 18O</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 </ins>BBa_J23106<ins class="diffchange diffchange-inline">] </ins>iGEM plate 1, 18O</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Inoculation of TY-medium for Glycerol Stocks'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Inoculation of TY-medium for Glycerol Stocks'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* GVP cluster <ins class="diffchange diffchange-inline">([http://partsregistry.org/wiki/index.php?title=Part:BBa_I750016 BBa_I750016]) in </ins>vector <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 </ins>BBa_J61035<ins class="diffchange diffchange-inline">] </ins>(2x) in ''E.coli TOP10''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Terminator <del class="diffchange diffchange-inline">vector </del>? (2x) in ''E.coli TOP10''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Terminator <ins class="diffchange diffchange-inline">([http://partsregistry.org/wiki/index.php</ins>?<ins class="diffchange diffchange-inline">title=Part:BBa_B0014 BBa_B0014]) in vector [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] </ins>(2x) in ''E.coli TOP10''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td></tr>
</table>Verhoeven1981http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=17089&oldid=prevJolandaWitteveen: /* Wet */2009-07-09T10:10:32Z<p><span class="autocomment">Wet</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:10, 9 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Purification of HmtA (PA Q9I147 6xHis Tag)''' </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Purification of HmtA (PA Q9I147 6xHis Tag)'''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml LB+amp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml LB+amp</div></td></tr>
</table>JolandaWitteveenhttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=17085&oldid=prevJolandaWitteveen: /* Wet */2009-07-09T10:09:47Z<p><span class="autocomment">Wet</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:09, 9 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Purification of HmtA (PA Q9I147 6xHis Tag) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''</ins>Purification of HmtA (PA Q9I147 6xHis Tag)<ins class="diffchange diffchange-inline">''' </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Preculture</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml LB+amp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* inoculaton of a colony in 5 ml LB+amp</div></td></tr>
</table>JolandaWitteveenhttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=17084&oldid=prevJolandaWitteveen: /* Wet */2009-07-09T10:09:23Z<p><span class="autocomment">Wet</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:09, 9 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Purification of HmtA (PA Q9I147 6xHis Tag) </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Preculture</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* inoculaton of a colony in 5 ml LB+amp</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Incubate overnight 37°C with shaking</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dry==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dry==</div></td></tr>
</table>JolandaWitteveenhttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=16506&oldid=prevJolandaWitteveen: /* Wet */2009-07-08T10:22:40Z<p><span class="autocomment">Wet</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:22, 8 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dry==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dry==</div></td></tr>
</table>JolandaWitteveenhttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=15391&oldid=prevJaspervdg: Dry work notes.2009-07-06T15:19:17Z<p>Dry work notes.</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:19, 6 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Dry==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We found that the dimensions given in Walsby1994 are in fact ''outer'' dimensions, instead of inner dimensions as we first assumed. Furthermore mass/density calculations were added to our computational model of the geometry of gas vesicles.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">It was found to be problematic to determine the buoyant density of E. coli with gas vesicles, as the volume of the bacteria can differ quite a bit (both from strain to strain and during the life cycle) and we also haven't found very concrete data about the number of gas vesicles produced (nor any direct measurements of the buoyant density of E. coli with gas vesicles). So instead we've decided to give a lower bound on the (volume) fraction of the cell that should consist of gas vesicles.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Groningen/Notebook/Day/Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Groningen/Notebook/Day/Footer}}</div></td></tr>
</table>Jaspervdghttp://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=15327&oldid=prevVerhoeven1981 at 13:16, 6 July 20092009-07-06T13:16:48Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:16, 6 July 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Groningen/Notebook/Day/Header}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Groningen/Notebook/Day/Header}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Wet==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The remainer was centrifuged for 1 min. at full speed and the cells were resuspended in 100ul TY-medium, and plated on TY-Agar-Amp plated. The plates were stored at 37C o.n.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The remainer was centrifuged for 1 min. at full speed and the cells were resuspended in 100ul TY-medium, and plated on TY-Agar-Amp plated. The plates were stored at 37C o.n.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Inoculation of TY-medium <del class="diffchange diffchange-inline">with </del>Glycerol Stocks'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Inoculation of TY-medium <ins class="diffchange diffchange-inline">for </ins>Glycerol Stocks'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10''</div></td></tr>
</table>Verhoeven1981http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_July_2009&diff=15324&oldid=prevVerhoeven1981: New page: {{Team:Groningen/Notebook/Day/Header}} '''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106''' * BBa_J23109 iGEM plate 2, 2G * BBa_J23100 iGEM plate 1, 18C *...2009-07-06T13:13:37Z<p>New page: {{Team:Groningen/Notebook/Day/Header}} '''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106''' * BBa_J23109 iGEM plate 2, 2G * BBa_J23100 iGEM plate 1, 18C *...</p>
<p><b>New page</b></p><div>{{Team:Groningen/Notebook/Day/Header}}<br />
<br />
'''Transformation of ''E. coli TOP10'' with BBa_J23109, BBa_J23100 and BBa_J23106'''<br />
<br />
* BBa_J23109 iGEM plate 2, 2G<br />
* BBa_J23100 iGEM plate 1, 18C<br />
* BBa_J23106 iGEM plate 1, 18O<br />
<br />
All dry plasmid DNA in the wells was dissolved in 15ul sterile diH2O, and transfered to 500ul cups.<br />
<br />
* Four cups with 50ul competent ''E.coli TOP10'' cells were thawed on ice, and 2ul of dissolved plasmid was added.<br />
* After 30 min. on ice, the cups were put in a heat block at 37C for 5 min. and followed by 5 min. on ice.<br />
* To the cells, 800ul TY medium was added and incubated at 37C for 1 hour.<br />
* 100ul was plated on TY-Agar-Amp plates and incubated o.n. at 37C.<br />
* The remainer was centrifuged for 1 min. at full speed and the cells were resuspended in 100ul TY-medium, and plated on TY-Agar-Amp plated. The plates were stored at 37C o.n.<br />
<br />
'''Inoculation of TY-medium with Glycerol Stocks'''<br />
<br />
* GVP cluster vector BBa_J61035 (2x) in ''E.coli TOP10''<br />
* Terminator vector ? (2x) in ''E.coli TOP10''<br />
<br />
* 4.5 ml TY-medium with Amp. was inoculated with a colony grown on TY-Agar-Amp. plates o.n.<br />
* The tubes were put in a waterbath at 37C (150 rpm) and grown o.n. <br />
<br />
{{Team:Groningen/Notebook/Day/Footer}}</div>Verhoeven1981