Team:Groningen/Notebook/9 July 2009
From 2009.igem.org
(Difference between revisions)
(→Wet) |
(→Wet) |
||
Line 7: | Line 7: | ||
* Make a 12% SDS-gel | * Make a 12% SDS-gel | ||
* Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders | * Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders | ||
- | * Run | + | * Run 2h at 100V |
+ | * Stain the gel in coomassie blue. | ||
+ | |||
'''Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation''' | '''Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation''' |
Revision as of 12:05, 9 July 2009
Wet
Purification of HmtA (PA Q9I147 6xHis Tag)
- SDS-gel electrophosis
- Make a 12% SDS-gel
- Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders
- Run 2h at 100V
- Stain the gel in coomassie blue.
Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation
- BBa_J23109 (labelled as no.1) vector BBa_J61002 in E.coli TOP10
- BBa_J23100 (labelled as no.2) vector BBa_J61002 in E.coli TOP10 (red colony)
- BBa_J23106 (labelled as no.3) vector BBa_J61002 in E.coli TOP10 (red colony)
- The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul).
- The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|