Team:Groningen/Notebook/9 July 2009
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(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== '''Purification of HmtA (PA Q9I147 6xHis Tag)''' * SDS-gel electrophosis * Make a 12% SDS-gel * Fill gel with samples Wash 1-2 20mM imidazo...) |
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* Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders | * Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders | ||
* Run 1h at 100V | * Run 1h at 100V | ||
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+ | '''New Colonies''' | ||
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+ | The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul). The tubes were placed in a 37C shaking waterbath over night. | ||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} |
Revision as of 11:31, 9 July 2009
Wet
Purification of HmtA (PA Q9I147 6xHis Tag)
- SDS-gel electrophosis
- Make a 12% SDS-gel
- Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders
- Run 1h at 100V
New Colonies
The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul). The tubes were placed in a 37C shaking waterbath over night.
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